ZFIN ID: ZDB-FIG-151001-31
Percival et al., 2015 - Variations in sister chromatid cohesion dysfunction in esco2 mutant zebrafish reflects the phenotypic diversity of Roberts Syndrome. Disease models & mechanisms   8(8):941-55 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. S3 CRISPR/Cas9 derived esco2 null allele recapitulates esco2 retroviral insertion mutant. (A) Brightfield images of 24 hpf esco2+13/+13 allele recapitulates embryonic lethality and head necrosis (n=41 with head necrosis and 137 WT morphology) of esco2m/m. (B) Allele diagram of esco2 +13 allele. A 13 bp insertion occurs in the second ATG containing exon of the 11 exon gene. ATG denotes the transcriptional start site and TAA denotes translation stop site. Blue text indicates CRISPR target site, the PAM is boxed, and red text indicates 13 bp insertion. Codons separated by spaces, note alternative codons in the +13 allele due to the frame shift. (C) Metaphase chromosome spreads from 24 hpf pooled (n=30) esco2+13/+13 embryos. Percentage distribution of each category: “paired”, “paired but separated” (PBS), and “separated”. Representative spreads and diagrams to describe category shown above percentages (n=30 spreads).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
esco2uab1/uab1 standard conditions Prim-5 cell chromosome separated from cell chromosome, abnormal
Prim-5 head necrotic, abnormal
Prim-5 sister chromatid cohesion decreased process quality, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.