FIGURE
Fig. S4
- ID
- ZDB-FIG-150804-7
- Publication
- Panza et al., 2015 - Live imaging of endogenous protein dynamics in zebrafish using chromobodies
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Fig. S4
PCNA-CB and human PCNA-GFP dynamically colocalize when expressed in live zebrafish. 36 hpf hsp70l:PCNA-CB embryos transiently expressing human PCNA-GFP from an injected DNA construct. (a) Overview of the scanned area. (b) Detailed time lapse analysis. Live imaging of animals heat-shocked at 24 hpf shows that PCNA-CB and human PCNA-GFP mark the same nuclear structures and their pattern is characteristic of S phase. Additionally, both fluorescent fusions can trace these structures’ dynamics (arrowheads and arrows indicate two different cells over time). Scale bars: 20 µm. Timestamps: h:min:s. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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