FIGURE

Fig. 9

ID

ZDB-FIG-150615-9

Publication

Krock et al., 2014 - The Par-PrkC Polarity Complex Is Required for Cilia Growth in Zebrafish Photoreceptors

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Fig. 9

Photo-Morpholinos cause rhodopsin mislocalization and loss of rod photoreceptors. (A, E) Transverse retinal cryosections from 4 dpf wild-type; (B, F) pard3MO-pard3pMO; (C, G) prkciMO-prkcipMO; (D, H) prkci/zMO-prkci/zpMO injected larvae. Embryos were injected with MO/S-photo-MO hybrids and irradiated at 30 hpf with UV light. Immunohistochemistry was performed on transverse cryosections of 4 dpf larvae and stained with 1D1 to label rhodopsin (green) and phalloidin (red) and counterstained with DAPI to label nuclei. Bottom row shows 1D1 and DAPI staining only. (I) Quantification of total 1D1+ rod photoreceptors in 10 micron cryosections. (*** = p≤0.0001) ONL = outer nuclear layer; INL = inner nuclear layer. Scale bar = 100 μm (A–D) or 20 μm (E–H).

Expression Data

Gene:	rho
Antibody:	Ab1-rho
Fish:	AB/EKW AB/EKW + MO1-pard3ab AB/EKW + MO1-prkci AB/EKW + MO1-prkci + MO1-prkcz
Knockdown Reagents:	MO1-pard3ab MO1-prkci MO1-prkcz
Anatomical Terms:	retinal rod cell cell body retinal rod cell photoreceptor inner segment retinal rod cell photoreceptor outer segment
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/EKW + MO1-pard3ab AB/EKW + MO1-prkci AB/EKW + MO1-prkci + MO1-prkcz
Knockdown Reagents:	MO1-pard3ab MO1-prkci MO1-prkcz
Observed In:	camera-type eye photoreceptor cell differentiation photoreceptor cell photoreceptor outer segment retina retinal rod cell
Stage:	Day 4

Phenotype Detail

Acknowledgments

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