FIGURE

Fig. 1

ID

ZDB-FIG-150608-1

Publication

Hermkens et al., 2015 - Sox7 controls arterial specification in conjunction with hey2 and efnb2 function

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Fig. 1

Disrupted blood circulation in sox7 zebrafish mutants. (A) sox7 in situ hybridization of 3dpf wild-type embryos (left panel, lateral view; right panel, dorsal view) showing sox7 expression in all main vessels. (B) Schematic diagram of the sox7^hu5626 allele with a premature stop-codon after amino acid 53 (red asterisk). HMG, high mobility group box; TAD, trans-activating domain. (C) Overall normal appearance of sox7^hu5626 heterozygous sibling and homozygous mutant at 2dpf. (D) On average, 59% of sox7^hu5626 mutants display disturbed blood flow at 2.5dpf. Percentages of pooled embryos from four independent experiments (total of 275 embryos). Percentages can vary substantially between different backgrounds. (E) kdrl:GFP;gata1:dsRed-positive sox7^hu5626 mutants lack functional blood circulation in the trunk, whereas heterozygous siblings have normal circulation at 2.5dpf. Right panels: higher magnifications of boxed area.

Expression Data

Genes:	DsRed EGFP sox7
Fish:	WT sox7^hu5626/+; s843Tg; sd2Tg sox7^{hu5626/hu5626}; s843Tg; sd2Tg
Anatomical Terms:	blood dorsal aorta head blood vessel posterior cardinal vein trunk blood vessel vasculature
Stage Range:	Pec-fin to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sox7^{hu5626/hu5626} sox7^{hu5626/hu5626}; s843Tg; sd2Tg
Observed In:	blood circulation trunk blood circulation
Stage:	Pec-fin

Phenotype Detail

Acknowledgments

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