FIGURE

Fig. 4

ID

ZDB-FIG-150514-2

Publication

Hou et al., 2014 - Simultaneous mapping of membrane voltage and calcium in zebrafish heart in vivo reveals chamber-specific developmental transitions in ionic currents

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Fig. 4

Figure 4. Voltage and calcium transients of the atrium and ventricle in an embryonic zebrafish heart during development. Top: Fluorescence images of zebrafish hearts expressing CaViar showed transition of the heart tube into two chambers. Scale bars 50 μm. Bottom: Dual-wavelength imaging of CaViar reported voltage (red), and calcium (blue) transients in the atrium (lighter shade) and ventricle (darker shade) at (A) 36 hpf, (B) 54 hpf, (C) 102 hpf. At 36 hpf, low expression of Arch(D95N) led to higher noise than at other time-points. Blue illumination intensity was minimized at all time-points to minimize photo-inactivation of blebbistatin. This occasionally led to increased noise in the GCaMP5G fluorescence (e.g., at 102 hpf).

Expression Data

Gene:	GCaMP
Fish:	a300Tg (AB)
Anatomical Terms:	heart heart tube
Stage Range:	Prim-25 to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Physiol.