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Sumoylation of C/ebpα is involved in HSPC development. (A) The expression of cebpa was examined by RT-PCR analysis. EGFP positive cells were sorted from the Tg (cmyb-EGFP) and Tg (gata1-EGFP) transgenic lines at 72 hpf, respectively, and subjected to RT-PCR. As a control, transcripts of the bactin gene were amplified. P.C., positive control (plasmid). N.C., negative control (H2O). (B–F) WISH assay of cmyb at 30 hpf. Boxed regions indicate the AGM. (B′–F′) Magnified images of corresponding boxed regions from B to F, respectively. Red arrows identify cmyb-positive cells in the AGM. (G–I) Fluorescent images of the Tg (cmyb-EGFP) line at 72 hpf. (G′–I′) Magnified images of corresponding boxed regions from G to I, respectively. White arrows identify cmyb-positive cells in the CHT. (J–M) WISH assay of cmyb in the sae1 mutant embryos and siblings at 72 hpf. (N–R) WISH assay of cmyb in the cebpa mutant embryos and siblings at 72 hpf. (J′–R′) Magnified images of corresponding boxed regions from J to R, respectively. Blue arrows identify cmyb-positive cells in the CHT.
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