FIGURE

Fig. 7

ID

ZDB-FIG-150416-13

Publication

Cheng et al., 2015 - Nephron proximal tubule patterning and corpuscles of Stannius formation are regulated by the sim1a transcription factor and retinoic acid in zebrafish

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Fig. 7

The inhibition of RA biosynthesis by DEAB induces distal fates but fails to rescue CS formation in sim1a morphants. DMSO control or 1.67×10⁵ M DEAB treatments of (A) wild-types or (B) sim1a morphant embryos from 75% epi to the 5–7 ss were performed. WISH analysis in embryos at 24 hpf for distal segment markers (slc12a1, stc1, and slc12a3) (purple) and the somites (smyhc1) (red). Black bars represent segment domains in correlation to somite numbers. Embryo anterior is located to the left. Insets show dorsal view of the CS. Abbreviations: CS – corpuscles of Stannius; DE – distal early; DEAB – 4-diethylaminobenzaldehyde; DL – distal late; epi – epiboly; hpf – hours post fertilization; ss – somite stage; WISH – whole mount in situ hybridization.

Expression Data

Genes:	slc12a1 slc12a3 stc1
Fish:	TU TU + MO1-sim1a
Conditions:	chemical treatment: drug chemical treatment: 4-(diethylamino)benzaldehyde
Knockdown Reagent:	MO1-sim1a
Anatomical Terms:	corpuscles of Stannius pronephric distal early tubule pronephric distal late tubule
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU TU + MO1-sim1a
Conditions:	chemical treatment: drug chemical treatment: 4-(diethylamino)benzaldehyde
Knockdown Reagent:	MO1-sim1a
Observed In:	corpuscles of Stannius pronephric distal early tubule pronephric distal late tubule pronephric nephron tubule development pronephros
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 399(1), Cheng, C.N., Wingert, R.A., Nephron proximal tubule patterning and corpuscles of Stannius formation are regulated by the sim1a transcription factor and retinoic acid in zebrafish, 100-16, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.