Fig. 1

Generation of the transgenic zebrafish with the NTR-EGFP expression under the control of zebrafish dnd promoter.

A) genomic structure of zebrafish dnd gene, including 5′UTR region, 4 exons and 3′ UTR region identified for its PGC-specific presence in the fourth exon. The positions for the region in zebrafish DNA sequence BX890575.23 are labeled in blue. The position sites for the region used related to the transcriptional starting site of dnd are labeled in black. B) schematic representation of the transgenic construct containing the 5′-dnd:ntr-egfp-3′UTR of dnd-3′ fragment flanked by I-Sce1 sites. C) Schematic representation of the transgenic construct containing the 5′-dnd:ntr-egfp without dnd-3′ fragment flanked by I-Sce1 sites. D) Fluorescence microscopic image showing EGFP-expressing PGCs of a F0 progeny at 72 hpf. E) in situ hybridization signals of vasa probe showing the position of PGC at 72 dpf.