FIGURE

Fig. 1

ID

ZDB-FIG-150413-8

Publication

Ma et al., 2015 - Jag1b is essential for patterning inner ear sensory cristae by regulating anterior morphogenetic tissue separation and preventing posterior cell death

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Fig. 1

jag1b gene expression is blocked in the gene-trapping jag1b mutant. (A) EGFP expression patterns in jag1b^+/- embryos. EGFP is expressed in the otic vesicle (arrows). Scale bar: 100µm. (B) The zebrafish jag1b genomic locus. Primers used in D are indicated. (C) The mature wild-type (WT) jag1b transcript and the predicted mutant jag1b-EGFP fusion transcript. Primers used in E are indicated. (D) PCR genotyping confirms that the jag1b gene is disrupted in jag1b mutants (right). Wild type, heterozygotes and homozygotes show different GFP intensity (left). (E) RT-PCR analysis confirms the existence of the jag1b-EGFP fusion transcript in jag1b^+/- and jag1b^-/- embryos. (F,G) Real-time RT-PCR (F) and western blot (G) analyses indicate that jag1b mRNA and protein levels are decreased in jag1b mutant embryos. Error bars indicate s.e.m. of triplicated experiments.

Expression Data

Gene:	EGFP
Fish:	jag1b^gd2Gt/+
Anatomical Terms:	head otic vesicle
Stage Range:	Sphere to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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