ZFIN ID: ZDB-FIG-150413-23
Gu et al., 2015 - Defective apical extrusion signaling contributes to aggressive tumor hallmarks. eLIFE   4:e04069 Full text @ Elife
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Day 5

Fig. 7

(A) Representative confocal projections of 5-dpf WT (left) and Mil (S1P2 mutant) (right) zebrafish larvae treated with DMSO or FAK inhibitor PF573228, where high Texas-Red Dextran indicates poor permeability in Mil but is greatly reduced when barrier function is improved with FAK inhibitor treatment. Scale bar = 10 µm and red box indicates region of fish imaged. (B) 5 dpf Mil and wild type zebrafish treated with and without FAK inhibitor. Note that while FAK inhibitor-treated Mil have other developmental defects (heart and circulation), there are no obvious clumps as seen in the untreated fish. Scale bar = 100 µm and red box indicates region of fish imaged. Note FAK inhibitor does not affect WT zebrafish. (C) Quantification of epidermal masses in 5 dpf Mil zebrafish larvae with and without PF573228. (D) Quantification of apoptotic cells within epidermal masses with and without PF573228. For both, error bars are SD and p values are ***<0.0001. (E) Immunoblot showing knockdown of S1P2 by photo-activatable morpholinos. (F) Stills from a video where PF573228 was added to S1P2 morphant at 5 dpf, where red arrows show the edge of the epidermis over time, scale bar = 50 µm and red box indicates region of fish imaged. Time is hours:minutes following FAK inhibitor addition. Note: epidermal cells that are sloughed off become embedded in the agarose where fish is mounted.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-s1pr2 control Day 5 epidermal cell aggregated, abnormal
WT + MO1-s1pr2 chemical treatment: EC 2.7.10.2 (non-specific protein-tyrosine kinase) inhibitor Day 5 epidermal cell cell aggregation decreased amount, abnormal
s1pr2m93/m93 chemical treatment: EC 2.7.10.2 (non-specific protein-tyrosine kinase) inhibitor Day 5 epidermal cell cell aggregation decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife