Fig. 6
F-actin in microridges is highly dynamic. Photoconversion of actin-mEos was performed on 3 dpf larvae by focusing on a sub-region (red circle) of an epithelial cell displaying microridges in vivo and time-lapse images were taken before (labeled with blue frame) and after photoconversion. Upon photoconversion, mEos-actin is irreversibly converted from emitting a green to a red fluorescent signal. For details of photoconversion refer to Materials and Methods. Single plane of confocal image of red channel, green channel and overlay of the two are shown. Data are representative of 9 individual cells. Scale bar, 10 µm. See S7 Movie. |