ZFIN ID: ZDB-FIG-141006-12
Boczonadi et al., 2014 - EXOSC8 mutations alter mRNA metabolism and cause hypomyelination with spinal muscular atrophy and cerebellar hypoplasia. Nature communications   5:4287 Full text @ Nat. Commun.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 8

(a) Representative graph shows the survival rate of un-injected, mpba+p53, exosc8+p53 and combined mbpa+exosc8+p53 MO injections of the Tg(islet-1:GFP) strain at 48 hpf (summary of 3 experiments). The survival rate increased after triple MO injections compared with exosc8+p53 knockdown embryos. (b) Top row: morphology of the severe embryos at 48 hpf injected with exosc8+p53 and exosc8+mbpa+p53. Bottom row: brain abnormalities of exosc8+p53 and exosc8+mbpa+p53 morphants (Tg(islet-1:GFP) strain) at 48 hpf. Dorsal views of GFP positive neurons in the midbrain and the hindbrain indicate abnormal brain structures in severe exosc8+p53 morphants. Improved cranial neuron structure was detected in severe mbpa+exosc8+p53 MO injected embryos. The same result has been reproduced in three separate experiments with equal MO doses, on the same clutch of embryos.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
rw0Tg + MO1-exosc8 + MO1-mbpa + MO4-tp53 standard conditions Long-pec brain development disrupted, abnormal
Long-pec hindbrain morphology, abnormal
Long-pec midbrain morphology, abnormal
rw0Tg + MO1-exosc8 + MO4-tp53 standard conditions Long-pec brain development disrupted, abnormal
Long-pec hindbrain morphology, abnormal
Long-pec midbrain morphology, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.