FIGURE

Fig. 3

ID

ZDB-FIG-140923-27

Publication

Lin et al., 2014 - Hypoxia-Inducible Factor 2 Alpha Is Essential for Hepatic Outgrowth and Functions via the Regulation of leg1 Transcription in the Zebrafish Embryo

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Fig. 3

Hif2-alpha plays a major role in liver development.

The specificity of knockdown by MO was demonstrated by monitoring lfabp gene expression at 5 pdf by WISH in wild-type embryos (A) and embryos injected with hif2-alpha ATG-MO (B). The small liver phenotype resulting from the knockdown of hif2-alpha can be rescued by co-injection of hif2-alpha mRNA (C). ATG-MO of hif2-alpha but not hif1-alpha caused the small liver phenotype (E). ATG-MO of hif3-alpha also caused a slight effect on liver development (F).

Expression Data

Gene:	fabp1a
Fish:	WT WT + MO1-epas1b WT + MO1-hif1al WT + MO2-hif1ab
Knockdown Reagents:	MO1-epas1b MO1-hif1al MO2-hif1ab
Anatomical Term:	hepatocyte
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-epas1b
Knockdown Reagent:	MO1-epas1b
Observed In:	liver
Stage:	Day 5

Phenotype Detail

Acknowledgments

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