FIGURE

Fig. S4

ID

ZDB-FIG-140811-28

Publication

Zaghloul et al., 2010 - Functional analyses of variants reveal a significant role for dominant negative and common alleles in oligogenic Bardet-Biedl syndrome

Other Figures

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Fig. S4

Rescue of bbs morphants with human BBS mRNA. Translation blocking morpholinos for bbs1-12 were injected into zebrafish embryos and produced early embryonic defects including shortened body axis and abnormal somite and notochord morphology. These defects could be rescued with co-injection of human WT mRNA encoding the respective BBS gene. Co-injection with mRNA baring mutations producing a spectrum of effects ranging from rescue similar to WT rescue, such as BBS6 R518H (R), or partial rescue, as with the Hypomorph BBS1 M390R (C), or lack of rescue, such as the Null BBS10 Y197C (D′), or exacerbation of the phenotype, such as the Dominant negative BBS3 G169A (I).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-bbs1 WT + MO1-bbs4 WT + MO1-bbs5 WT + MO1-bbs7 WT + MO1-bbs10 WT + MO1-bbs12 WT + MO1-mkks WT + MO1-ttc8 WT + MO3-bbs2 WT + MO3-bbs9 WT + MO4-arl6
Knockdown Reagents:	MO1-bbs1 MO1-bbs4 MO1-bbs5 MO1-bbs7 MO1-bbs10 MO1-bbs12 MO1-mkks MO1-ttc8 MO3-bbs2 MO3-bbs9 MO4-arl6
Observed In:	gastrulation notochord somite whole organism anterior-posterior axis
Stage Range:	5-9 somites to 10-13 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA