FIGURE

Fig. S3

ID

ZDB-FIG-140619-16

Publication

Smith et al., 2013 - A compact, in vivo screen of all 6-mers reveals drivers of tissue-specific expression and guides synthetic regulatory element design

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Fig. S3

(a) Stable F1 transgenics were generated by breeding wildtype Ab females with F0 males expressing 2CD12/epidermis, 2GH08/brain, 2GH12/notochord, and 3CD05/spinal cord. In each case, the F1 fish exhibited the original expression pattern (white arrows). (b) For each of the four tissue-specific multiplexed constructs, the E1b minimal promoter was replaced by a 31bp TATA-box containing minimal promoter from the pGL4.23 vector (Promega). In every case, except 2GH12/notochord, the pGL minimal promoter constructs exhibited the same tissue expression pattern (white arrows) as the original multiplexed sequence.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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