FIGURE

Fig. 6

ID
ZDB-FIG-140522-11
Publication
Nissim et al., 2014 - Prostaglandin E2 regulates liver versus pancreas cell-fate decisions and endodermal outgrowth
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Fig. 6

PGE2 Interacts with the bmp2b Pathway to Regulate Liver versus Pancreas Specification

(A) Modulation of PGE2 levels from 12 to 20 hpf impacts expression of bmp2b in the lateral plate mesoderm as shown by in situ hybridization at 20 hpf. dmPGE2 increases bmp2b expression (n = 15/19 embryos), whereas Indo decreases bmp2b expression (n = 14/21 embryos). Lower: magnified view of the area in the dashed square. bmp2b is also expressed in the otic vesicles (arrow).

(B and C) Size of pancreas-specified population marked by pdx1 is impacted by modulation of the PGE2 or bmp2b pathways. Representative views are shown in (B), and quantification is shown in (C). dmPGE2 or heat-shock activation of bmp2b diminishes whereas Indo, dorsomorphin (Dorso), or heat-shock activation of dnBmpR promotes the pancreas progenitor population. The impact of dmPGE2 is blunted by Dorso or dnBmpR, and the impact of Indo is blunted by heat-shock activation of bmp2b. (Data are represented as mean ± SEM; n e 10 per condition; t test comparisons: p < 0.05 comparing treatment versus control; #no significant difference comparing with versus without dmPGE2; ##no significant difference comparing with versus without Indo.)

Expression Data
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage: 20-25 somites

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Conditions:
Observed In:
Stage: 20-25 somites

Phenotype Detail
Acknowledgments
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Reprinted from Developmental Cell, 28(4), Nissim, S., Sherwood, R.I., Wucherpfennig, J., Saunders, D., Harris, J.M., Esain, V., Carroll, K.J., Frechette, G.M., Kim, A.J., Hwang, K.L., Cutting, C.C., Elledge, S., North, T.E., and Goessling, W., Prostaglandin E2 regulates liver versus pancreas cell-fate decisions and endodermal outgrowth, 423-437, Copyright (2014) with permission from Elsevier. Full text @ Dev. Cell