ZFIN Figure: Ogryzko et al., 2014, Fig. 3

ZFIN ID: ZDB-FIG-140507-17

ZFIN ID: ZDB-FIG-140507-17

Ogryzko et al., 2014 - Zebrafish tissue injury causes up-regulation of interleukin-1 and caspase dependent amplification of the inflammatory response. Disease models & mechanisms 7(2):259-64 Full text @ Dis. Model. Mech.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Gene:	EGFP
Fish:	sh235Tg
Conditions:	transection: caudal fin chemical treatment by environment: EC 3.4.22.36 (caspase-1) inhibitor, transection: caudal fin
Anatomical Term:	caudal fin
Stage:	Protruding-mouth

PHENOTYPE:

Fish:	sh235Tg
Conditions:	transection: caudal fin chemical treatment by environment: EC 3.4.22.36 (caspase-1) inhibitor, transection: caudal fin
Observed In:	caudal fin
Stage:	Protruding-mouth

Fig. 3

Caspase-1 inhibitor YVAD downregulates NF-κB activation in response to injury. (A) Fluorescence photomicrograph of pNF-κB:EGFP embryos following tailfin transection at 1 hpi (i) and 8 hpi (ii) indicating the region quantified (square bracket). Scale bars: 100 μm. (B) There is a 2.4-fold increase in EGFP fluorescence in response to injury, quantifiable as average fluorescent intensity across the transection site (indicated in A). ****Pd0.0001 by t-test. (C) Embryos treated with 50 μM YVAD show a reduction in EGFP fluorescence at 8 hpi compared with DMSO-treated controls and embryos treated with qVD. **Pd0.01 by one-way ANOVA with Dunnett’s post-test. n=30 performed as three independent experiments.

Gene Expression Details

Gene	Antibody	Fish	Conditions	Stage	Anatomy	Assay
EGFP		sh235Tg	transection: caudal fin	Protruding-mouth	caudal fin	IFL
		sh235Tg	transection: caudal fin, chemical treatment by environment: EC 3.4.22.36 (caspase-1) inhibitor	Protruding-mouth	caudal fin	IFL

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
sh235Tg	transection: caudal fin	Protruding-mouth	caudal fin EGFP expression increased amount, abnormal
sh235Tg	transection: caudal fin, chemical treatment by environment: EC 3.4.22.36 (caspase-1) inhibitor	Protruding-mouth	caudal fin EGFP expression amount, ameliorated

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.