FIGURE

Fig. 2

ID

ZDB-FIG-140422-16

Publication

Roberts et al., 2014 - Targeted transgene integration overcomes variability of position effects in zebrafish

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Fig. 2

Variability of position effects on reporter activity is sharply reduced among PhiC31 integrase-mediated transgenic lines compared with Tol2 transgenesis. (A) Schematic of the pTol2/EL161-krt4:Venus construct injected with Tol2 transposase mRNA. Below are examples of expression patterns of transgenic F1 larvae (3 dpf). TP indicates Tol2-mediated expression patterns. Numbers refer to domains of Venus expression activity (listed in Fig. 3B). (B) Schematic indicates the recombination of the targeting plasmid pattB-mCherry,Tol2/EL161-krt4:Venus into the attP docking site of the transgenic recipient line Tg(Xla.crygc:attP-GFP)uobL6. Larvae from three different founders (IF1, IF2, IF8) targeted with PhiC31 integrase are shown. Venus expression domains are labelled as in Fig. 3B. Lens activity driven by mCherry (arrowheads in bottom row) demonstrates PhiC31-mediated integration. Insert in bottom right shows brightfield image of the head region. Arrows labelled ‘ysl’ in red channel indicate auto-fluorescence of the yolk syncytial layer. Dorsal views of larvae head are shown. Scale bar: 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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