Fig. 6

CHIA expression patterns at the larval stage; days 4 and 5. (A–D) 4 DPF larvae, (E) 5 DPF larvae. All bars represent 1 mm. (A) 4 DPF panel of all CHIA gene expression patterns. Expression in intestinal tissues become increasingly visible and is especially clear in the stomach for CHIA.1 (A1), and the liver for CHIA.5 and CHIA.6 (A5–6). (B) Lateral 4 DPF head close-up. The white matter (WM) can be distinguished clearly over the generally diffuse staining in the brain. (C) 6 μm section through the head of an embryo at 4 DPF. The white matter (WM) staining is outlined, and the pharyngeal arches (PA) and several head blood vessels (HBV) are also clearly discernable. Underneath is a schematic representation showing the approximate position of the slice in the embryo. (D) Dorsal 4 DPF head close-up. The habenulae (H) can be clearly distinguished in CHIA.4. (E) 5 DPF panel of all CHIA genes. CHIA.1, CHIA.2 and CHIA.3 (E1–3) are all nearly exclusively expressed in the lining of the stomach (S). CHIA.4, CHIA.5 and CHIA.6 much less pronounced so (E4–6). Some major blood vessels of the head are still visible such as the primordial midbrain channel (PMBC in E5). CHIA.6 seems to be primarily expressed in the liver (L in E6).