FIGURE

Fig. 1

ID

ZDB-FIG-140421-19

Publication

Zhang et al., 2014 - Quantitative phenotyping-based in vivo chemical screening in a zebrafish model of leukemia stem cell xenotransplantation

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Schematic representation of the experimental design for LSC inhibitor screening in zebrafish.

(A) Cultured K562 cells expressing a Kusabira-orange (KOr) fluorescent protein. Scale bar: 50 μm. (B) Sorting of ALDH- and ALDH+ cell populations. (C) A preparation of zebrafish embryos. Scale bar: 500 μm. (D) Xenotransplantation of ALDH- and ALDH+ cells into zebrafish at 48 hpf. Scale bar: 1.0 mm. (E, F) Xenografted zebrafish were transferred into 96-well plates at 72 hpf, imaged using a high-content imaging system, and then treated with the test compounds. Scale bar: 1.0 mm. (G) At 120 h (48 h after treatment), the xenografted zebrafish were imaged again to evaluate the effects of the chemicals. Scale bar: 1.0 mm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One