Fig. 2
- ID
- ZDB-FIG-140417-21
- Publication
- Bhatia et al., 2014 - A survey of ancient conserved non-coding elements in the PAX6 locus reveals a landscape of interdigitated cis-regulatory archipelagos
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Characterisation of a novel conserved element E+120 in mouse and zebrafish reporter transgenics. (A) The E+120 element (orange ellipse) is located 120 kb 3′ of the PAX6 P1 promoter in the human locus, in the large final intron of ELP4. (B) VISTA plot using the human sequence as a base, showing the deep conservation of E+120 in mouse, chicken, coelacanth, one of the zebrafish pax6 loci and the elephant shark. The constructs used for the reporter transgenic experiments are shown. (C) Four independent transgenic mouse lines were analysed for the E120Z construct, showing variable patterns of expression at E11.5. (D) At E17.5 strong expression is seen in the cerebella (black arrow) and olfactory bulbs (white arrowhead) in 3 out of 4 lines. (E) Expression pattern of the elephant shark E+120 element in transgenic zebrafish, shown by mCherry (CHR) fluorescence. Signal is seen in the olfactory placodes (op) and olfactory bulbs (ob) from 24 hpf. At 48 hpf (shown at two different confocal plains) and 96 hpf expression is also found at the midbrain-hindbrain boundary (mhb) and in the hindbrain (hb) region. |
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| Stage Range: | Prim-5 to Day 4 |
Reprinted from Developmental Biology, 387(2), Bhatia, S., Monahan, J., Ravi, V., Gautier, P., Murdoch, E., Brenner, S., van Heyningen, V., Venkatesh, B., and Kleinjan, D.A., A survey of ancient conserved non-coding elements in the PAX6 locus reveals a landscape of interdigitated cis-regulatory archipelagos, 214-228, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.