Fig. 7

Loss of Sufu further enhances Gli activity in MZkif7 mutants.

(A–D) Parasagittal optical sections of 30 hpf WT (A,B) or MZkif7(C,D) embryos stained with anti-Prox1 (red) and 4D9 (green) antibodies; morpholino mediated knock-down of sufu results in significant increase in Prox1 expressing slow twitch muscle cells in WT (B, quantified in I) and a further increase in MZkif7(D) mutants; Scale bar: 50 µm. (E–H) Lateral view of 30 hpf WT (A) or MZkif7(C) embryos hybridized with an antisense probe for ptch2 mRNA; MZkif7 embryo shows an expansion of ptch2 expression; embryos of similar stage and genotype injected with sufu MO result in an enhancement of the levels of ptch2 staining in WT (F) and in MZkif7 (H) embryos; Scale bar: 50 µm. (I) Quantification of Prox1⁺ slow twitch muscle fiber nuclei per hemisegment in WT, sufu MO injected, MZkif7 and MZkif7 injected with sufu MO. Error bars represent standard deviation obtained from 16–20 hemisegments from >6 embryos. Note the significant increase in MZkif7 compared to WT and a further enhancement in slow fibers by removal of Sufu in MZkif7 mutants.