FIGURE

Fig. 2

ID

ZDB-FIG-140305-61

Publication

Shimada et al., 2012 - A high-throughput fluorescence-based assay system for appetite-regulating gene and drug screening

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Gene expression and the effect of knocking down genes associated with appetite regulation.

(A) Analysis by qPCR of brain-derived neurotrophic factor mRNA (bdnf) during paramecia feeding, n = 5, *P<0.05. (B and C) Western blots showing embryonic injection of neuropeptide Y (npy) MO (B) and preproinsulin a (insa) MO. (D) Embryonic injection of npy MO and npy mRNA. Orexigenic gene knockdown decreased feeding volume. (E) insa MO and insa mRNA, orexigenic gene knockdown increased feeding volume at 7 dpf. n = 15, *P<0.05. All values are mean ± SEM. (F and G) Knockdown of npy (F) and insa (G) affected locomotor activities at 5 dpf. n = 16, *P<0.05. All values are mean ± SEM. (H) Detection of gene knockdown for splicing MOs in 2% (w/v) agarose gels stained with ethidium bromide.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-ins WT + MO1-npy
Knockdown Reagents:	MO1-ins MO1-npy
Observed In:	feeding behavior
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One