Fig. 4

NTF^ΔGPS mRNA rescues the trabeculation phenotype in Gpr126-depleted zebrafish. (A) Lateral view of control-, MO2-, or MO2+NTF^ΔGPS mRNA-injected embryos at 75 hpf. MO2 injection resulted in pericardial edema (arrow). (B) Confocal sections of the hearts from control-, MO2-, and MO2+NTF^ΔGPS mRNA-injected Tg(myl7:EGFP-HsHRAS)^s883 embryos at 80 hpf. In Gpr126-depleted animals, trabeculation (yellow arrowheads) is perturbed. Note that this phenotype is rescued by NTF^ΔGPS mRNA injection. (C) Quantitative analysis of rescue experiments of coinjections of MO2 and mRNAs encoding NTF^ΔGPS or NTF subfragments. Mean ± SEM. (D) Whole-mount in situ hybridization demonstrating krox20/egr2 and mbp expression in the otic vesicle (yellow arrow) and PLLn Schwann cells (black arrow) and krox20/egr2 expression in the hindbrain (arrowhead) at 75 hpf. In contrast, expression of krox20/egr2 and mbp is down-regulated in PLLn of MO2-mediated full-length Gpr126-depleted embryos. Coinjection of NTF^ΔGPS mRNA failed to rescue the PLLn myelineation phenotype in the cardiac phenotype-rescued animals. **P < 0.01.