FIGURE

Fig. 3

ID

ZDB-FIG-131105-18

Publication

Rosenfeld et al., 2013 - Small heat shock proteins Hspb7 and Hspb12 regulate early steps of cardiac morphogenesis

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Fig. 3

Hspb12 is required for normal numbers of ventricular cardiomyocytes. (A) Representative cardiac phenotypes observed at 48 hpf development in embryos derived from the myl7:gfp; myl7:dsRed-nls reporter strain stained with S46 antibody to differentiate the atrium (blue) from the ventricle (green). (B) Quantification of cardiomyocytes in wildtype embryos (wt, n=7) or embryos injected with standard control MO (control, n=7), hspb7 MO (hspb7, n=7), hspb12 MO (hspb12, n=8), or both hspb7 and hspb12 MOs (hspb7+12, n=6). ^Np<0.05 by Student′s t-test in comparison to standard control group. The bars represent 10 μm.

Expression Data

Genes:	DsRed EGFP myh6
Antibody:	Ab-S46
Fish:	f2Tg; twu34Tg f2Tg; twu34Tg + MO1-hspb7 f2Tg; twu34Tg + MO1-hspb12 f2Tg; twu34Tg + MO1-hspb12 + MO1-hspb7
Knockdown Reagents:	MO1-hspb7 MO1-hspb12
Anatomical Terms:	atrial myocardium cardiac muscle cell cardiac muscle cell
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	f2Tg; twu34Tg + MO1-hspb7 f2Tg; twu34Tg + MO1-hspb12 f2Tg; twu34Tg + MO1-hspb12 + MO1-hspb7
Knockdown Reagents:	MO1-hspb7 MO1-hspb12
Observed In:	atrial myocardium cardiac muscle cell cardiac muscle cell heart ventricular myocardium cardiac muscle cell
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 381(2), Rosenfeld, G.E., Mercer, E.J., Mason, C.E., and Evans, T., Small heat shock proteins Hspb7 and Hspb12 regulate early steps of cardiac morphogenesis, 389-400, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.