FIGURE

Fig. 2

ID

ZDB-FIG-131002-23

Publication

Riesco et al., 2013 - Cryopreservation Causes Genetic and Epigenetic Changes in Zebrafish Genital Ridges

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Fig. 2

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Fig. 2

Relative expression of zebrafish genes after CPAs exposure (CPAs) and cryopreservation (Cryo).

Cryopreservation affects gene expression levels in zebrafish genital ridges. Analysis was carried out in zebrafish genital ridges (GRs) containing primordial germ cells (PGCs). Expression levels for each gene relative to actb2 were calculated for all samples using the 2^-ΔΔCt method. All results were expressed as the means ± SE of the 2^-ΔΔCt method of three independent experiments with three replicates for each. The Student’s t-test (µ=1) was performed to identify changes in gene expression levels after cryopreservation. Asterisks showed significant (p< 0.05) downregulation after cryopreservation (Cryo).

Expression Data

Genes:	cxcr4b ddx4 dnd1 hsp70l hsp90ab1 pou5f3 sox2 sox3
Fish:	zf45Tg
Anatomical Term:	primordial germ cell
Stage:	26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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