FIGURE

Fig. 1

ID
ZDB-FIG-130906-51
Publication
Glenn et al., 2013 - Analysis of Gpr126 function defines distinct mechanisms controlling the initiation and maturation of myelin
Other Figures
All Figure Page
Back to All Figure Page
Fig. 1

Schwann cell membrane wrapping, compaction, and the maturation of the myelin sheath proceed in gpr126 mutant nerves after transient elevation of cAMP. (A-C2) TEM images of transverse sections through zebrafish larvae at 9 dpf showing the ultrastructure of the PLLn one week following a pulse treatment with either DMSO (A,B) or 50 μM FSK (C). (A) gpr126st49/+ control DMSO-treated fish have normal myelination in the PLLn. (B) The PLLn of gpr126st49/st49 mutant larvae has no myelinated axons at 9 dpf. (C) The PLLn of gpr126st49/st49 mutant larvae that were treated with FSK from 50 to 55 hpf contains some rescued myelinated axons. A2-C2 show higher magnification TEM images of the boxed regions in A-C, respectively. (D-F2) TEM images of transverse sections through juvenile zebrafish at 30 dpf showing the ultrastructure of the PLLn four weeks following a pulse treatment with either DMSO (D,E) or 50 μM FSK (F). (D) gpr126st49/+ control DMSO-treated fish have normal myelination in the PLLn at 30 dpf. (E) The PLLn of gpr126st49/st49 mutant fish has no myelinated axons at 30 dpf. (F) The PLLn of gpr126st49/st49 mutant fish that were treated with FSK from 50 to 55 hpf contains a subset of rescued myelinated axons. D2-F2 show higher magnification TEM images of the boxed regions in D-F, respectively. (G,H) Quantification of the number of myelinated axons present per PLL nerve in heterozygous (H), mutant (M) and FSK-treated mutant (M+FSK) nerves at 9 dpf (G) and 30 dpf (H). Error bars represent s.d. Significance with two-tailed Student’s t-test: ***P<0.001, **P<0.01. Scale bars: 1 μm in A-C; 0.5 μm in A2-C2,D2-F2 2 μm in D-F.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Condition:
Observed In:
Stage Range: Days 7-13 to Days 30-44

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development