ZFIN ID: ZDB-FIG-130730-7
Wilfinger et al., 2013 - Cell type and tissue specific function of islet genes in zebrafish pancreas development. Developmental Biology   378(1):25-37 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Prim-15 to Day 4
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. 7 TUNEL and EdU assays on control and mutant embryos. (A–D) Confocal image projections of 30 hpf (A+B) and 48 hpf (C+D) old control and isl1 mutant embryos in the tg[hb9:GFP] background after TUNEL labeling for apoptotic cells. The arrow highlights individual TUNEL+ cells in the vicinity of the GFP labeled islet. No TUNEL+ cells were found within the pancreatic islet of control or mutant fish at all analyzed stages. (E+F) Confocal sections of 96 hpf embryos labeled for Ins (in red) and EdU (in blue). Embryos were treated with EdU from 48 hpf to 96 hpf. Note that very few EdU+ cell are present within the islet (highlighted by yellow circle), while most of the nuclei in the surrounding exocrine tissue are EdU-positive. All images show ventral view, anterior to the left. (G) Numbers of Ins+ and Ins+/EdU+ cells in control and isl1 mutants. (H) Percentage of EdU+/Ins+ cells in relation to the number of Ins+ cells. Bars show mean+SEM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP isl1asa29/sa29; ml2Tg standard conditions Prim-15 posterior pancreatic bud IHC
Long-pec endocrine pancreas IHC
ml2Tg standard conditions Prim-15 posterior pancreatic bud IHC
Long-pec endocrine pancreas IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-ins IHC WT standard conditions Day 4 endocrine pancreas insulin secreting cell
IHC isl1asa29/sa29 standard conditions Day 4 endocrine pancreas insulin secreting cell
Phenotype Details
Fish Conditions Stage Phenotype
isl1asa29/sa29 standard conditions Day 4 endocrine pancreas lacks parts or has fewer parts of type insulin secreting cell, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 378(1), Wilfinger, A., Arkhipova, V., and Meyer, D., Cell type and tissue specific function of islet genes in zebrafish pancreas development, 25-37, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.