FIGURE

Fig. 2

ID
ZDB-FIG-130404-23
Publication
Doganli et al., 2012 - The alpha2Na+/K+-ATPase is critical for skeletal and heart muscle function in zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. 2

α2-MO-mediated Atp1a2 knockdown affects skeletal and heart muscle. (A) Embryos injected with α2-MO or p53-MO+7alpha;2-MO exhibited a contorted tail, abnormal somite morphology, a swollen pericardium and loss of cardiac laterality. Coinjecting α2-MO with Atp1a2 mRNA rescued knockdown phenotypes demonstrating specificity. Normal and distorted somite borders of std-MO- and α2-MO-injected embryos are indicated by dashed lines in the bottom panel. (B) Heart rates (beats/min) of wild-type (WT), std-MO- and α2-MO-injected embryos at 60 hpf (n = 30) (see supplementary material Movies 1, 2). (C) The penetrance of the observed phenotypes in α2-MO-injected (n = 91) and mRNA-rescued embryos (n = 136), plotted as percentages ± s.d. of embryos displaying the phenotype, relative to total embryo number. (D) Embryos injected with Ncx4a-MO phenocopy the α2-MO-injected embryos. (E) The penetrance of the observed phenotypes in Ncx4a-MO-injected embryos (n = 107), plotted as percentages ± s.d. of embryos displaying the phenotype, relative to total embryo number. (F) Heart rates (beats/min) of WT and bradycardic Ncx4a-MO-injected embryos at 60 hpf (n = 20). *P<0.05, ****P<0.0001.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.