FIGURE

Fig. S5

ID
ZDB-FIG-130311-14
Publication
Ye et al., 2013 - S1pr2/Gα13 signaling controls myocardial migration by regulating endoderm convergence
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Fig. S5

Cardiac-specific expression of Gα13a fails to rescue cardia bifida caused by global Gα13 inhibition. The transgene myl7:gna13a-IRES-nlsGFP was created using the Gateway system (Kwan et al., 2007; Villefranc et al., 2007), such that Gα13a is expressed specifically in the myocardial cells under control of the myl7 promoter (Huang et al., 2003). The expression of Gα13a was monitored as nuclear GFP (nlsGFP) expression driven by an internal ribosomal entry site (IRES) (Kwan et al., 2007). The transgene plasmid DNA (50 pg) was co-injected with transposase RNA (30 pg) into the cytoplasm of wild-type embryos at the one-cell stage. A stable line expressing Gα13a in the heart was identified. Injections of gnb13ab MOs in Tg(myl7:gna13a-IRES-nlsGFP) embryos still exhibited cardia bifida. (A) A representative epifluorescence image of 30 hpf-Tg(myl7:gna13a-IRES-nlsGFP) embryos, showing expression of nlsGFP in the heart (arrow). (B) A confocal Z-stack image showing that nlsGFP is expressed in all cardiomyocytes of Tg(myl7:gna13a-IRES-nlsGFP) embryos at 30 hpf. (C) A representative epifluorescence image of 30 hpf-Tg(myl7:gna13a-IRES-nlsGFP) embryos injected with gna13ab MOs showing cardia bifida (arrows). (D) Frequencies of cardia bifida in 30-hpf embryos. #P>0.5 versus control. Scale bar: 100 μm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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