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SET8 regulates Wnt target genes in zebrafish. (A and B) Knockdown of set8a resulted in dorsal mesodermal expansion. Embryos injected with the indicated MOs were fixed at the shield stage and stained for tbx6, cdx4, gsc, and ntl. The graph shows the statistical data for each gene, respectively. (C) set8a MO showed synergistic effect with wnt8 MOs on Wnt responsive genes. The graph shows the statistical data of the in situ experiment for each gene, respectively. (D) Set8a regulated cdx4 and tbx6 expression. The embryos injected MOs, as indicated, were collected at the shield stage. The cdx4 and tbx6 expression was detected by quantitative real-time PCR and normalized by ntl gene expression. (E) set8a MO and wnt8 MOs reduced the transcription level of cdx4 and tbx6, both alone and synergistically. **P < 0.01; *P < 0.05.
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