FIGURE

Fig. 3

ID

ZDB-FIG-130212-3

Publication

Wang et al., 2013 - Wdr68 requires nuclear access for craniofacial development

Other Figures

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Fig. 3

Fusion of Wdr68 to the Mad repression domain is not tolerated in vivo.

A-E) Ventral views of alcian blue stained animals with the Meckel’s (M), palatoquadrate (PQ) and ceratohyal (CH) labeled in red. A) Wildtype sibling. Red arrow indicates the M-PQ jaw joint. B) Severe craniofacial defects in wdr68^hi3812 animals and failure of GFP mRNA to rescue. C) Rescue of the severe craniofacial defects by injection of FlagWdr68 (FW) mRNA. Red arrow indicates the mild M-PQ joint fusion phenotype. D) Severe craniofacial defects in wdr68^hi3812 animals and failure of MadFlagWdr68 (MFW) mRNA to rescue. 52/52 mutants genotyped were wdr68^hi3812 homozygotes. E) Rescue of the severe craniofacial defects by injection of CebpFlagWdr68 (CFW) mRNA. Red arrow indicates the mild M-PQ joint fusion phenotype. F) Graph summarizing the results of four injection trials. In aggregate, n = 112/452 (25%) for GFP animals, n = 16/352 (4.5%) for FW animals, n = 96/296 (32%) for MFW animals, and n = 2/149 (1.3%) for CFW animals.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dcaf7^{hi3812Tg/hi3812Tg}
Observed In:	Meckel's cartilage palatoquadrate cartilage
Stage:	Day 4

Phenotype Detail

Acknowledgments

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