ZFIN ID: ZDB-FIG-130210-17
Bercier et al., 2013 - WNK1/HSN2 Mutation in Human Peripheral Neuropathy Deregulates KCC2 Expression and Posterior Lateral Line Development in Zebrafish (Danio rerio). PLoS Genetics   9(1):e1003124 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. S2

Motor neurons of wnk1/hsn2 knockdown embryos are not morphologically abnormal. Wnk1/hsn2 knockdown embryos expressing GFP under the HB9 promoter (Tg(mnx1:GFP) from Flanagan-Street et al., 2005) were obtained for all three AMOs conditions (A-non injected embryo, B- MO-wnk1-ATG, C- MO-hsn2-SB52, D- MO-hsn2-SB32). The primary motor neurons did not show any defects at 48hpf, which further parallels the HSANII pathology, where patients have no motor dysfunction.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP ml2Tg standard conditions Long-pec primary motor neuron axon IFL
Long-pec primary motor neuron neuronal cell body IFL
ml2Tg + MO1-wnk1b standard conditions Long-pec primary motor neuron axon IFL
Long-pec primary motor neuron neuronal cell body IFL
ml2Tg + MO2-wnk1b standard conditions Long-pec primary motor neuron axon IFL
Long-pec primary motor neuron neuronal cell body IFL
ml2Tg + MO3-wnk1b standard conditions Long-pec primary motor neuron axon IFL
Long-pec primary motor neuron neuronal cell body IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ml2Tg + MO1-wnk1b standard conditions Long-pec motor neuron axon guidance process quality, normal
Long-pec primary motor neuron axon morphology, normal
ml2Tg + MO2-wnk1b standard conditions Long-pec motor neuron axon guidance process quality, normal
Long-pec primary motor neuron axon morphology, normal
ml2Tg + MO3-wnk1b standard conditions Long-pec motor neuron axon guidance process quality, normal
Long-pec primary motor neuron axon morphology, normal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.