ZFIN ID: ZDB-FIG-130210-14
Bercier et al., 2013 - WNK1/HSN2 Mutation in Human Peripheral Neuropathy Deregulates KCC2 Expression and Posterior Lateral Line Development in Zebrafish (Danio rerio). PLoS Genetics   9(1):e1003124 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 3

WNK1/HSN2 knockdown leads to abnormal neuromast development.

A) The number of structural hair cells was assessed using transgenic embryos expressing GFP under the beta-tubulin promoter, revealing only the neuronal hair cells within PLL neuromasts. C) The number of functional hair cells was assessed by using transgenic embryos expressing GFP under the claudin-b promoter, rendering the neuromast fluorescent. The functional hair cells were revealed by incubation in the styryl dye FM-464FX, shown in red. B, D) Hair cells were counted for each PLL neuromast and totals were tabulated in box plots showing that WNK1/HSN2 knockdown embryos have a significantly lower number of structural and functional hair cells within their neuromasts when compared with non-injected embryos. The knockdown embryos presented in (A) and (C) are representative results at 72hpf obtained from MO-hsn2-SB32 injection. The number of neuromasts counted per condition is indicated in the boxes and the total number of embryos obtained per condition is indicated in parenthesis at the bottom of the box plots. Scale bar: 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP zf106Tg standard conditions Protruding-mouth posterior lateral line neuromast plasma membrane IFL
zf106Tg + MO3-wnk1b standard conditions Protruding-mouth posterior lateral line neuromast plasma membrane IFL
GFP mi2Tg standard conditions Protruding-mouth posterior lateral line neuromast neuromast hair cell IFL
mi2Tg + MO3-wnk1b standard conditions Protruding-mouth posterior lateral line neuromast neuromast hair cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
mi2Tg + MO3-wnk1b standard conditions Protruding-mouth posterior lateral line neuromast development decreased process quality, abnormal
Protruding-mouth posterior lateral line neuromast has fewer parts of type neuromast hair cell, abnormal
zf106Tg + MO1-wnk1b standard conditions Protruding-mouth posterior lateral line neuromast development decreased process quality, abnormal
Protruding-mouth posterior lateral line neuromast has fewer parts of type neuromast hair cell, abnormal
zf106Tg + MO2-wnk1b standard conditions Protruding-mouth posterior lateral line neuromast development decreased process quality, abnormal
Protruding-mouth posterior lateral line neuromast has fewer parts of type neuromast hair cell, abnormal
zf106Tg + MO3-wnk1b standard conditions Protruding-mouth posterior lateral line neuromast development decreased process quality, abnormal
Protruding-mouth posterior lateral line neuromast has fewer parts of type neuromast hair cell, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.