FIGURE

Fig. 3

ID

ZDB-FIG-130117-50

Publication

Chu et al., 2012 - Yolk syncytial layer formation is a failure of cytokinesis mediated by Rock1 function in the early zebrafish embryo

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Fig. 3

Disruption of cytoskeletal elements induces formation of enlarged YSL.

(A) Control, (B) nocodazole-treated, (C) cytochalasin D-treated, and (D) Y27632-injected embryos between oblong to sphere stages. Arrows in Bi–Di indicate enlarged YSL. Curly brackets in Aii–Dii indicate the width of the YSL microtubule belt. (Aiii–Diii) High magnification images of boxes in Aii–Dii respectively. (Aii,Aiii) Microtubule distribution in a control embryo. A large aster in the YSL (stippled circle, Aiii). (Bii,Biii) Disorganized microtubules in the YSL and YCL, with small asters in the enlarged YSL (arrowheads, Biii). (Cii,Ciii) Gaps in the YCL (Ciii, asterisks). (Dii,Diii) Dense YSL and sparse YCL microtubule arrays. The enlarged syncytia express mtx1 (Biv–Div), and induces ntl in the overlying blastoderm (Bv–Dv). (E) Mesoderm induction assay. The enlarged syncytium (curly bracket) induced ntl in the fluorescein-labelled animal cap.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB
Conditions:	chemical treatment: cytochalasin D chemical treatment: nocodazole
Observed In:	internal yolk syncytial layer microtubule yolk syncytial layer yolk syncytial layer aster yolk syncytial layer microtubule
Stage Range:	Oblong to Sphere

Phenotype Detail

Acknowledgments

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