ZFIN ID: ZDB-FIG-121030-40
Shwartz et al., 2012 - Muscle contraction controls skeletal morphogenesis through regulation of chondrocyte convergent extension. Developmental Biology   370(1):154-163 Full text @ Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 5

Fig. 1 Muscle contraction is necessary for craniofacial morphogenesis. (A) Left: Lateral view of control and tricaine-paralyzed zebrafish embryos at 120 hpf, using bright field microscopy. Right: Ventral view of flat-mounted Alcian blue stained control and tricaine-paralyzed zebrafish. (B) Left: Lateral view using bright field microscopy of control and nic mutant 120 hpf embryos. Lateral view (middle) and flat-mounted ventral view (right) of control and nic mutant zebrafish stained with Alcian blue. (C) Left: Lateral view using bright field microscopy of control and myf5/myod double morphant zebrafish at 120 hpf. Lateral view (middle) and flat-mounted ventral view (right) of control and myf5/myod embryos stained with Alcian blue. Asterisk indicates pericardial edema (A, B). Arrows indicate lower jaw (A)–(C). Double-headed arrows indicate relative location between Meckel′s and ceratohyal cartilages and between Meckel′s cartilage and the neurocranium (B, C). (D) Graph showing the reduction in ceratohyal length in paralyzed zebrafish compared to control (p<0.0001). Both right and left ceratohyal cartilages from twelve tricaine-paralyzed, eight nic mutant and a corresponding number of control embryos were measured at 120 hpf. (E) Graph showing the increase in the angle between ceratohyal cartilage and the midline (dashed line in A–C) in tricaine-paralyzed and nic mutant embryos compared to control (p<0.0001). The angle of both right and left ceratohyal cartilage of 6 tricaine-paralyzed and 11 nic mutants at 120 hpf was measured. Scale bars represent 100 μm. Abbreviations: m: Meckel′s cartilage, ch: ceratohyal cartilage, cb: ceratobranchials, pq: palatoquadrate, nc: neurocranium.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-myod1 + MO3-myf5 standard conditions Day 5 ceratohyal cartilage decreased length, abnormal
Day 5 embryonic cranial skeleton morphogenesis disrupted, abnormal
chrna1b107/b107 standard conditions Day 5 ceratohyal cartilage decreased length, abnormal
Day 5 embryonic cranial skeleton morphogenesis disrupted, abnormal
Day 5 pericardium edematous, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 370(1), Shwartz, Y., Farkas, Z., Stern, T., Aszódi, A., and Zelzer, E., Muscle contraction controls skeletal morphogenesis through regulation of chondrocyte convergent extension, 154-163, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.