ZFIN ID: ZDB-FIG-121011-28
Kizil et al., 2012 - The chemokine receptor cxcr5 regulates the regenerative neurogenesis response in the adult zebrafish brain. Neural Development   7(1):27 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
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Anatomical Terms:
Stage: Adult

Fig. S2 Neurons express cxcr5. (A) cxcr5 fluorescent in situ hybridization (FISH) coupled to HuC immunohistochemistry (IHC) on a section of unlesioned adult zebrafish telencephalon. Inset is magnified image. (B)cxcr5 FISH coupled to HuC IHC on a section of 3 days post lesion (dpl) adult zebrafish telencephalon. Inset is magnified image. cxcr5 is expressed in neurons before and after injury. (C) Quantification graph for cxcr5-expressing HuC-positive cells. (D)cxcr5 chromogenic in situ hybridization (ISH) coupled to NeuroD IHC in unlesioned telencephalon. NeuroD-positive differentiating neurons, which are several cell diameters away from the ventricle express cxcr5 (white asterisks). cxcr5 expression is weaker in NeuroD-positive neurons in comparison to cxcr5-positive cells closer to the ventricle (yellow asterisks). In a transition zone between strong NeuroD-positive (white asterisks) and NeuroD-negative cells (yellow asterisks), cells express NeuroD and cxcr5 weakly (blue asterisks). (E)cxcr5 chromogenic ISH coupled to NeuroD IHC in 3 dpl telencephalon. NeuroD-positive differentiating neurons are more numerous, dispersed inside the parenchyma distantly in comparison to unlesioned telencephalons and express cxcr5 (white asterisks). cxcr5 expression is weaker in NeuroD-positive neurons in comparison to cxcr5-positive cells closer to the ventricle (yellow asterisks). (F) Quantification graph for cxcr5-positive NeuroD-expressing cells. The number of NeuroD/cxcr5 double-positive cells increase upon injury in adult zebrafish telencephalon. Scale bars 25 μm; n = 4 telencephalons for every set of analyses.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cxcr5 WT control Adult telencephalon neuron ISH
Adult telencephalon radial glial cell ISH
WT physical alteration: anatomical structure Adult telencephalon neuron ISH
Adult telencephalon radial glial cell ISH
knu3Tg control Adult telencephalon neuron ISH
Adult telencephalon radial glial cell ISH
knu3Tg physical alteration: anatomical structure Adult telencephalon neuron ISH
Adult telencephalon radial glial cell ISH
EGFP knu3Tg control Adult telencephalon neuron IHC
knu3Tg physical alteration: anatomical structure Adult telencephalon neuron IHC
neurod1 WT control Adult telencephalon neuron IHC
WT physical alteration: anatomical structure Adult telencephalon neuron IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neural Dev.