FIGURE

Fig. 3

ID

ZDB-FIG-120918-10

Publication

Cunningham et al., 2012 - Identification of benzodiazepine Ro5-3335 as an inhibitor of CBF leukemia through quantitative high throughput screen against RUNX1-CBFbeta interaction

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Fig. 3

Ro5-3335 rescues hematopoietic defect in the RUNX1–ETO transgenic embryos. (A) RUNX1-ETO transgenic embryos at 48 h postfertilization (hpf). HS, heat shock. Arrows point to the posterior blood island. Note the pooling of blood cells in the heat-shocked embryo. (B) Heat-shocked RUNX1-ETO transgenic embryos at 48 hpf that were treated with either 25 μM Ro5-3335 (Upper) or DMSO (Lower). (C) Bar graphs showing the effect of incubating heat-shocked RUNX1-ETO transgenic embryos with Ro5-3335 at the indicated concentrations. Black bars, embryos with blood pooling and no circulation; gray bars, embryos without blood pooling but also no circulation; white bars, embryos without blood pooling and with circulating cells. Means of three independent experiments are shown with SDs. *P < 0.03; **P < 0.003.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA