ZFIN Image: Cunningham et al., 2012, Fig. 3

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Figure Caption

Fig. 3 Ro5-3335 rescues hematopoietic defect in the RUNX1–ETO transgenic embryos. (A) RUNX1-ETO transgenic embryos at 48 h postfertilization (hpf). HS, heat shock. Arrows point to the posterior blood island. Note the pooling of blood cells in the heat-shocked embryo. (B) Heat-shocked RUNX1-ETO transgenic embryos at 48 hpf that were treated with either 25 μM Ro5-3335 (Upper) or DMSO (Lower). (C) Bar graphs showing the effect of incubating heat-shocked RUNX1-ETO transgenic embryos with Ro5-3335 at the indicated concentrations. Black bars, embryos with blood pooling and no circulation; gray bars, embryos without blood pooling but also no circulation; white bars, embryos without blood pooling and with circulating cells. Means of three independent experiments are shown with SDs. *P < 0.03; **P < 0.003.

Acknowledgments

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