FIGURE

Fig. S3

ID

ZDB-FIG-120720-28

Publication

Huang et al., 2012 - Attenuation of notch and hedgehog signaling is required for fate specification in the spinal cord

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Fig. S3

Activation of Notch signaling by NICD does not induce the expression of gli genes. hsp-Gal4; UAS-NICD embryos and non-double-transgenic sibling controls were treated with DMSO or cyclopamine at 4.3 hpf, heat-shocked at 11 hpf, and stained at 24 hpf for the expression of gli1, gli2a, gli2b, and gli3. Note that in cyclopamine-treated embryos (right panels), NICD-overexpressing embryos have similar level of expression of gli gene as control embryos, indicating that Notch signaling does not induce gli transcription in the absence of Hh signaling. Lateral views of embryos are shown. Scale bar: 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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