ZFIN ID: ZDB-FIG-120611-17
Manfroid et al., 2012 - Zebrafish sox9b is crucial for hepatopancreatic duct development and pancreatic endocrine cell regeneration. Developmental Biology   366(2):268-278 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Conditions:
Anatomical Terms:
Stage: Day 6
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Day 6

Fig. 6

sox9b is required for late endocrine cell formation in the IPD and for islet beta cell recovery upon ablation in larvae. (A–D) Immunodetection at 6 dpf of endocrine cell differentiation (Pax6) along the IPD labelled with 2F11 in wild type (A) and sox9b mutant larvae (B). Larvae were treated with DMSO (A, B) or DAPT (C, D) from 3 to 6 dpf. Secondary isolated endocrine cells are detected (small arrowheads) in wild type and sox9b mutant control larvae. The dotted line demarcates the intestine from the pancreatic region. (C, D) The massive increase in secondary islets resulting from DAPT treatment of wild type larvae (large arrowheads in C) is not observed in sox9b mutants which still display isolated endocrine cells along the IPD (small arrowheads in D). (E–H) Beta cell recovery in Tg(ins:nfsB-mCherry); sox9b larvae upon Metronidazole (Met) mediated ablation. Pancreatic ducts were labelled with 2F11 and beta cells were revealed by Insulin (Ins) immunodetection as mCherry fluorescence was undetectable after the process of immunofluorescence. (E, F) 6.5 dpf Tg(ins:nfsB-mCherry) wild type (E) and sox9b mutant larvae (F) 3 days (80 h) after DMSO treatment from 56 to 80 hpf. (G, H). Tg(ins:nfsB-mCherry) wild type (G) and sox9b mutant (H) larvae 3 days after Met exposure. Scale bar=20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ins jh4Tg control Day 6 endocrine pancreas IHC
jh4Tg chemical treatment: metronidazole Day 6 endocrine pancreas IHC
sox9bfh313/fh313; jh4Tg control Day 6 endocrine pancreas IHC
sox9bfh313/fh313; jh4Tg chemical treatment: metronidazole Day 6 endocrine pancreas IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-2F11 IHC WT control Day 6 pancreatic duct
IHC WT chemical treatment: DAPT Day 6 pancreatic duct
IHC jh4Tg control Day 6 pancreatic duct
IHC jh4Tg chemical treatment: metronidazole Day 6 pancreatic duct
IHC sox9bfh313/fh313 control Day 6 pancreatic duct
IHC sox9bfh313/fh313 chemical treatment: DAPT Day 6 pancreatic duct
IHC sox9bfh313/fh313; jh4Tg control Day 6 pancreatic duct
IHC sox9bfh313/fh313; jh4Tg chemical treatment: metronidazole Day 6 pancreatic duct
Ab2-pax6 IHC WT control Day 6 endocrine pancreas
IHC Day 6 pancreatic duct endocrine cell
IHC WT chemical treatment: DAPT Day 6 endocrine pancreas
IHC Day 6 pancreatic duct endocrine cell
IHC sox9bfh313/fh313 control Day 6 endocrine pancreas
IHC Day 6 pancreatic duct endocrine cell
IHC sox9bfh313/fh313 chemical treatment: DAPT Day 6 endocrine pancreas
IHC Day 6 pancreatic duct endocrine cell
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: DAPT Day 6 pancreatic duct has extra parts of type endocrine cell, abnormal
Day 6 type B pancreatic cell differentiation increased process quality, abnormal
jh4Tg chemical treatment: metronidazole Day 6 endocrine pancreas has normal numbers of parts of type insulin secreting cell, normal
Day 6 type B pancreatic cell differentiation process quality, normal
sox9bfh313/fh313 control Day 6 pancreatic duct has normal numbers of parts of type endocrine cell, normal
sox9bfh313/fh313 chemical treatment: DAPT Day 6 pancreatic duct has normal numbers of parts of type endocrine cell, normal
sox9bfh313/fh313; jh4Tg chemical treatment: metronidazole Day 6 endocrine pancreas has fewer parts of type insulin secreting cell, abnormal
Day 6 type B pancreatic cell differentiation decreased process quality, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 366(2), Manfroid, I., Ghaye, A., Naye, F., Detry, N., Palm, S., Pan, L., Ma, T.P., Huang, W., Rovira, M., Martial, J.A., Parsons, M.J., Moens, C.B., Voz, M.L., and Peers, B., Zebrafish sox9b is crucial for hepatopancreatic duct development and pancreatic endocrine cell regeneration, 268-278, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.