FIGURE

Fig. 7

ID

ZDB-FIG-120525-8

Publication

Mattes et al., 2012 - Wnt3 and Wnt3a are required for induction of the mid-diencephalic organizer in the caudal forebrain

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Fig. 7

Lack of Wnt signaling leads to apoptosis in the MDO. Lateral views and dorsal views of the left hemisphere (marked by ′) of Shh::RFP transgenic embryos at 36 hpf. Wnt3/wnt3a morphant embryos as well as embryos treated with IWR1 display an increase of apoptotic cells within the MDO at 36 hpf (n = 4/6, A-C′). Upon blockage of Tp53 function apoptosis is down-regulated within the organizer tissue of wnt3/wnt3a morphant embryos as well as embryos treated with IWR1 and consistently, shh:RFP expression is restored (D-F′).

Expression Data

Gene:	DsRed
Fish:	ka300Tg ka300Tg + MO4-tp53 ka300Tg + MO1-wnt3 + MO2-wnt3a ka300Tg + MO1-wnt3 + MO2-wnt3a + MO4-tp53
Condition:	chemical treatment: IWR-1-endo
Knockdown Reagents:	MO1-wnt3 MO2-wnt3a MO4-tp53
Anatomical Term:	mid diencephalic organizer
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ka300Tg ka300Tg + MO4-tp53 ka300Tg + MO1-wnt3 + MO2-wnt3a ka300Tg + MO1-wnt3 + MO2-wnt3a + MO4-tp53
Condition:	chemical treatment: IWR-1-endo
Knockdown Reagents:	MO1-wnt3 MO2-wnt3a MO4-tp53
Observed In:	mid diencephalic organizer
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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