ZFIN ID: ZDB-FIG-120423-22
Arkhipova et al., 2012 - Characterization and regulation of the hb9/mnx1 beta-cell progenitor specific enhancer in zebrafish. Developmental Biology   365(1):290-302 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Long-pec

Fig. 1

Motoneuron and beta-cell specific GFP expression in Tg[hb9:GFP]ml2 embryos. (A) Schematic representation of the hb9 gene from different species. Highlighted are conserved non-coding regions (dark green), and exons (coding sequences in grey, untranslated regions in blue). (B–I2) In situ hybridization analysis comparing mRNA expression of hb9 (B, D, D2) and gfp (C, E, E2) in Tg[hb9:GFP]ml2 embryos at 17 hpf (B, C) and 24 hpf (D, E) reveals similar pattern in motoneurons and ventral interneurons (B–E, white arrows) and pancreas (B, C, white arrowheads). Asterisk in C marks ectopic gfp expression in rhombomere. D2, E2 show different focal planes of the embryos shown in D, E. (F–I) Confocal image projections of Tg[hb9:GFP]ml2 embryos labelled with GFP (green) and islet antibodies (red) at 16 hpf (F), 19 hpf (G) and 24 hpf (H) show overlapping expression of GFP and islet proteins in motoneurons (arrows) and in a subset of endocrine cells (arrowheads). (H–I2) Confocal image stacks of Tg[hb9:GFP]ml2 embryos labelled with antibodies for GFP and pancreatic hormones at 24 hpf (H2) and 48 hpf (I, I2) show that GFP overlaps with insulin (blue in H2, I) but not with glucagon (Glu) and somatostatin (Sst) (Glu + Sst in red, I2). Embryos are shown in dorsal (B–E) or ventral (F–I) view with anterior to the left. Scale bars in all figures correspond to 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP Ab3-GFP ml2Tg standard conditions 14-19 somites endocrine cell IHC
14-19 somites interneuron ISH
14-19 somites motor neuron ISH
Ab3-GFP 14-19 somites motor neuron IHC
14-19 somites pancreas primordium ISH
Prim-5 interneuron ISH
Prim-5 motor neuron ISH
Ab3-GFP Prim-5 pancreatic B cell IHC
Prim-5 pancreatic bud ISH
mnx1 AB standard conditions 14-19 somites interneuron ISH
14-19 somites motor neuron ISH
14-19 somites pancreas primordium ISH
Prim-5 interneuron ISH
Prim-5 motor neuron ISH
Prim-5 pancreatic bud ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-gcg IHC ml2Tg standard conditions Long-pec pancreatic A cell
Ab2-isl IHC ml2Tg standard conditions 14-19 somites endocrine cell
IHC 14-19 somites motor neuron
IHC Prim-5 endocrine cell
Ab3-ins IHC ml2Tg standard conditions Prim-5 pancreatic B cell
IHC Long-pec pancreatic B cell
Ab3-sst IHC ml2Tg standard conditions Long-pec pancreatic D cell
Phenotype Details No data available
Acknowledgments:
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Reprinted from Developmental Biology, 365(1), Arkhipova, V., Wendik, B., Devos, N., Ek, O., Peers, B., and Meyer, D., Characterization and regulation of the hb9/mnx1 beta-cell progenitor specific enhancer in zebrafish, 290-302, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.