FIGURE

Fig. S4

ID

ZDB-FIG-120416-8

Publication

Ninov et al., 2012 - Different levels of Notch signaling regulate quiescence, renewal and differentiation in pancreatic endocrine progenitors

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Fig. S4

Validation of Tg(hsp:ZdnSu(H)-myc; hsp:GFP) as a tool for downregulation of Notch signaling. (A) Embryos obtained from in-crossing (Tg(hsp:ZdnSu(H)-myc; hsp:GFP) parents were heat shocked at 24 hpf, fixed at 30 hpf and stained for Myc. The majority of the cells in the neural tube co-express Myc and GFP (projection of several planes). (B-D) Embryos obtained from crossing Tg(hsp:ZdnSu(H)-myc; hsp:GFP) and Tg(Tp1:H2BmCherry) parents were heat shocked at 12 hpf and examined at 30 hpf for GFP and Tg(Tp1:H2BmCherry) expression. (B) A single plane through the neural tube. GFP⁺ cells show no or low expression of Tg(Tp1:H2BmCherry), indicating that they had impaired capacity to activate Notch signaling. To visualize the cells better, high-magnification single planes are shown in B2-B222. White arrowheads indicate individual Tg(Tp1:H2BmCherry)⁺ cells. Yellow arrowheads indicate individual GFP⁺ cells. Note that in most cases, the two cell types are mutually exclusive. Analogous observations were made in the brain (C) and retina (D). The images in A and C are dorsal views, anterior towards the top. The images in B and D are lateral views, anterior towards the top, ventral towards the right. Scale bars: 20 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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