FIGURE

Fig. 6

ID

ZDB-FIG-120201-5

Publication

Li et al., 2011 - Smyd1b_tv1, a Key Regulator of Sarcomere Assembly, Is Localized on the M-Line of Skeletal Muscle Fibers

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Fig. 6

Smyd1b_tv1-EGFP is localized on the M-line of zebrafish skeletal muscles.

Smyd1b_tv1-EGFP construct was injected into Myomesin-RFP (A, C, E) or wild type (B, D, F) zebrafish embryos at 1–2 cell stages. Smyd1b_tv1-EGFP localization was determined together with M-line marker (Myomesin-RFP) and A-band marker (Myosin heavy chain) at 96 hpf. A, B and C. Co-localization of Smyd1b_tv1-EGFP and Myomesin-RFP was observed in myofibers of the injected embryos. D, E and F. Immunostaining with anti-MyHC antibody (F59) shows the localization of Smyd1b_tv1-EGFP in the middle of the A-bands in myofibers of zebrafish embryos. Scale bar: 12 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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