FIGURE

Fig. 4

ID

ZDB-FIG-120201-3

Publication

Li et al., 2011 - Smyd1b_tv1, a Key Regulator of Sarcomere Assembly, Is Localized on the M-Line of Skeletal Muscle Fibers

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Fig. 4

Rescue of myofibril organization defect in smyd1b knockdown embryos by expression of Smyd1b_tv1-EGFP or Smyd1b_tv2-EGFP fusion protein.

A. DNA constructs encoding Smyd1b_tv1-EGFP, or Smyd1b_tv2-EGFP fusion proteins or EGFP control were generated and injected into zebrafish embryos. B and C. Myofibers expressing Smyd1_tv1-EGFP (B) or Smyd1_tv2-EGFP (C) was directly observed by GFP. D and E. Myosin thick filaments organization was determined by F59 antibody staining in Smyd1_tv1-EGFP (D) or Smyd1_tv2-EGFP (E) co-injected embryos. F and G. Double staining shows the colocalization of normal fibers with Smyd1_tv1-EGFP (F) or Smyd1_tv2-EGFP (G) expression. Scale bars: 20 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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