Fig. 8

Comparison of osteoclasts in zebrafish during skeletal development of the wild-type and panther mutant zebrafish. (A) Development of OCs in the CTSK-GFP transgenic zebrafish. Lateral views of 8.5 mm larvae at 23 dpf of the zebrafish heterozygous (left; pan+/-) and homozygous (right; pan-/-) for the c-fms mutation. Anterior to the left. Yellow arrowheads show the CTSK-GFP positive cells in the panther mutant. (B) Relative CTSK-GFP positive area. GFP positive areas in the quantitative analysis were measured by image J software (pan+/- n = 6, pan-/- n = 6; error bars indicate s.d.; *p < 0.001 vs. WT). (C–F) Skeletal structures of the wild-type and panther mutant adult zebrafish. Bony tissues of 11 month-old adult zebrafish were stained with Alizarin Red. (C) Lateral view of wild-type zebrafish vertebrae. Ribs were removed. Vertebrae that have 16 or 17 hemal arches were started at the site shown in * toward the caudal fin ray (shown black arrow). (D) Frontal views of individual vertebrae of the wild-type (upper images) and panther (lower images). (E) Lateral views of a vertebrae, anterior to the left. (F) Frontal views of the same vertebrae. Red asterisks show the neural canal; and the blue one, the hemal canal. In the panther mutant, the diameter of the neural (F, red asterisks) or hemal canal (F, blue asterisks) became narrow. (G) Quantitative analysis for the size of areas of neural (upper graph) and hemal (lower graph) canals measured by image J software (WT n = 3, pan-/- n = 3; error bars indicate s.e.m.; *p < 0.05 vs. WT). Scale bars: 500 μm (A, C, D, E, F).