ZFIN Figure: Zuniga et al., 2011, Fig. 5

ZFIN ID: ZDB-FIG-111128-63

ZFIN ID: ZDB-FIG-111128-63

Zuniga et al., 2011 - Gremlin 2 regulates distinct roles of BMP and Endothelin 1 signaling in dorsoventral patterning of the facial skeleton. Development (Cambridge, England) 138(23):5147-5156 Full text @ Development

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	dlx3b hand2 msx1a
Fish:	edn1^{tf216b/tf216b} kca4Tg el49Tg; kca4Tg edn1^{tf216b/tf216b}; el49Tg; kca4Tg
Condition:	heat shock
Anatomical Terms:	mandibular arch skeleton pharyngeal arch cranial neural crest cell pharyngeal arch 1 pharyngeal arch 1 central region
Stage:	Prim-25

Fig. 5 Bmp4 induces hand2 and msxe expression in the absence of Edn1. (A-P) Confocal sections of 36 hpf in situ hybridizations for hand2 (green) with dlx3b (red, A-H) or msxe (red, I-P) in control hsp70I:Gal4 (A,I), edn1^-/- mutant (E,M), UAS:Bmp4 (B-D,J-L) and edn1^-/-; UAS:Bmp4 (F-H,N-P) embryos. Increasing periods of Bmp4 heat-shock induction [1 minute at 21 hpf (B,F,J,N), 3 minutes at 21 hpf (C,G,K,O) and 4 hours from 20-24 hpf (D,H,L,P)] resulted in progressive recovery of hand2 and msxe but not dlx3b expression in edn1^-/- mutants. Consistent phenotypes were observed for the following: (A) n=55, (B) n=31, (C) n=15, (D) n=9, (E) n=8, (F) n=3, (G) n=4, (H) n=1, (I) n=37, (J) n=11, (K) n=6, (L) n=9, (M) n=20, (N) n=2, (O) n=3 and (P) n=2. Scale bar: 50 μm.

Gene Expression Details

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay
dlx3b	edn1^{tf216b/tf216b}	heat shock	Prim-25	Not Detected	pharyngeal arch 1 central region	ISH
	edn1^{tf216b/tf216b}; el49Tg; kca4Tg	heat shock	Prim-25	Not Detected	pharyngeal arch 1 central region	ISH
	el49Tg; kca4Tg	heat shock	Prim-25		mandibular arch skeleton	ISH
	kca4Tg	heat shock	Prim-25		pharyngeal arch 1 central region	ISH
hand2	edn1^{tf216b/tf216b}	heat shock	Prim-25	Not Detected	pharyngeal arch cranial neural crest cell	ISH
	edn1^{tf216b/tf216b}; el49Tg; kca4Tg	heat shock	Prim-25	Not Detected	pharyngeal arch cranial neural crest cell	ISH
	el49Tg; kca4Tg	heat shock	Prim-25		pharyngeal arch cranial neural crest cell	ISH
	kca4Tg	heat shock	Prim-25		pharyngeal arch cranial neural crest cell	ISH
msx1a	edn1^{tf216b/tf216b}	heat shock	Prim-25	Not Detected	pharyngeal arch 1	ISH
	edn1^{tf216b/tf216b}; el49Tg; kca4Tg	heat shock	Prim-25	Not Detected	pharyngeal arch 1	ISH
	el49Tg; kca4Tg	heat shock	Prim-25		pharyngeal arch 1	ISH
	kca4Tg	heat shock	Prim-25		pharyngeal arch 1	ISH

Antibody Labeling Details No data available

Phenotype Details No data available

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development