FIGURE

Fig. 2

ID
ZDB-FIG-111117-3
Publication
Seger et al., 2011 - Analysis of Pax7 expressing myogenic cells in zebrafish muscle development, injury, and models of disease
Other Figures
All Figure Page
Back to All Figure Page
Fig. 2

Expression of TgBAC(pax7a:GFP)i131 and TgBAC(pax3a:GFP)i150 recapitulates the patterns of the endogenous genes in the myotome. A–C: At 24 hours post fertilization (hpf), Pax7 protein (A) and TgBAC(pax7a:GFP)i131 expression (B) mostly coincide (C), arrowheads highlight cells detected with α-Pax7, but barely GFP+ve. Green fluorescent protein (GFP) expression is also detected in processes extending from the xanthophores (B, arrows). D–F: At 48 hpf, labeling with α-DP312 (Pax3/Pax7) (D) and GFP expression driven by TgBAC(pax3a:GFP)i150 (E) show significant overlap (F); some nuclei labeled strongly by DP312 show little or no GFP expression, consistent with some cells expressing only Pax7 (arrows, inserts). G–I: Increasingly medial planes through 5 days postfertilization (dpf) TgBAC(pax7a:GFP)i131 larvae: at a superficial level, expression is observed in xanthophores (arrowheads) and along the horizontal and vertical myosepta (arrows, G). H: More medially, Pax7a cells align closely to myofibers, stained with Phalloidin (actin, red; arrows). I: Intense GFP expression in dorsal spinal cord (long arrow), dorsal neural crest cells (arrowheads), and alongside myofibers (small arrows). J: Individual fibers (α-actinin, red) are labeled in 48 hpf TgBAC(pax7a:GFP)i150 reflecting perdurance of the GFP protein. K,L: TgBAC(pax3a:GFP)i150 at 6 dpf: expression persists in fibers (K) and remains in rounded cells alongside the fibers at 10 dpf (L). M: Quantification of Pax7+ve nuclei at 24 hpf to 5 dpf detected in fixed specimens with αPax7 mAb and in vivo pax7a:GFP+ve cells. Counts include only weakly stained or weakly expressing Pax7+ve cells per somite and exclude intensely stained elongated Pax7+ve xanthophores. N–P: Cross-sections of Tg(Pax7a:GFP)i131 show a Pax7a:GFP+ve cell fully surrounded by β-catenin (arrows) and persistence of GFP in an individual fiber (arrowhead) at 6 dpf. N,O are magnifications of the boxed area in P. Scale bars = 50 μm in A–L, 25 μm in N–P.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.