FIGURE

Fig. 3

ID

ZDB-FIG-111104-2

Publication

Yeh et al., 2011 - Ptenb Mediates Gastrulation Cell Movements via Cdc42/AKT1 in Zebrafish

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Fig. 3

ptenb MOs delays epibolic progression.

Knockdown of ptenb by MOs caused delay in epibolic movement in a dose-dependent manner. (A) Embryos were injected with standard MO (StdMO), tMO₁, 5-bp mismatched tMO₁ (mis-tMO1) and tMO₂ at designated amount, incubated to 10 hpf and the percentages of embryos at each epiboly stage are shown. (B) Embryos were injected with 15 ng of StdMO or tMO₁ in the absence or presence of ptenb mRNAs (50 pg), incubated and their degrees of epiboly progression are shown. Each treatment was repeated at least 6 times.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TU + MO1-ptenb AB/TU + MO2-ptenb
Knockdown Reagents:	MO1-ptenb MO2-ptenb
Observed In:	epiboly involved in gastrulation with mouth forming second
Stage:	Bud

Phenotype Detail

Acknowledgments

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